What is the difference between NanoDrop one and one C?
Both models have the patented microvolume sample retention capability (pedestal). In addition, the NanoDrop Onec has a built-in cuvette capability which allows for all the same pedestal applications plus cuvette measurements to support kinetics applications and dilute sample measurements.
Does NanoDrop use UV light?
NanoDrop products use a unique sample retention technology that allow UV-Vis measurements to be made from 1µL of sample with no cuvettes or dilutions.
What volume of sample is required for the NanoDrop?
Q: What are the sample size requirements for the NanoDrop Lite? A: Although 1 ul volumes are usually sufficient for most sample measurements, increasing the sample size to 2 ul will ensure proper column formation for samples with reduced surface tension properties.
Can I use a NanoDrop to measure protein concentration?
The NanoDrop 2000c spectrophotometer can also be used to measure uncharacterized protein solutions, cell lysates, and crude protein extracts using colorimetric assays.
Why do you need to blank the NanoDrop?
If you see any peaks in your recordings you should clean the Nanodrop once again and re-blank the machine. You need to ensure your blank is exactly that, a sample free from nucleic acids and contaminants. If not, your sample values will suffer.
What can I use as a blank for NanoDrop?
Raise the nanodrop pedestal arm and place a 2μl drop of distilled water on the analyzer. Lower the pedestal arm and hit OK to initialize the instrument. 4. You will now need to blank the instrument using a 2μl drop of whatever substance your nucleic acid is suspended in.
Do NanoDrop Lite Plus Instruments come calibrated?
Calibration All NanoDrop Lite Plus instruments are calibrated at the time of manufacture and include a Factory Test Report. Should recalibration ever become necessary, this procedure must be performed by Thermo Fisher Scientific or the authorized service providers at our dealers.
How do I load and print on the NanoDrop Lite?
When you hear the paper feed mechanism move and the Home screen appears on the NanoDrop Lite, the printer is ready to load the paper; push the end of the paper into the paper feed slot on the printer back cover. Paper is properly inserted when it engages the print head mechanism and comes out from the slot at the top of the printer.
How does the NanoDrop Lite measure absorbance?
Using Light Emitting Diode (LED) technology, the NanoDrop Lite measures the sample absorption at 260 and 280 nm and corrects for scattering and background effects in the sample with light from a reference wavelength at 365 nm. The A260 and A280 is calculated with respect to the baseline absorbance at 365 nm.
What type of LED source does NanoDrop Lite use?
The NanoDrop Lite uses LED sources centered at 260 and 280 nm. The selection of quality LEDs and narrow band filters combine to ensure the highest wavelength accuracy.