Is pBR322 a plasmid vector?
pBR322 is a plasmid and was one of the first widely used E. coli cloning vectors.
What kind of plasmid is pBR322 How big is it?
Plasmid: pBR322 Size is 4363 for Boehringer, BRL, Promega, Sigma. Size is 4362 for USB. Promoter P1 is artificially created by the ligation of two different DNA fragments to create pBR322.
Why is pBR322 a useful vector?
pBR322 is a good cloning vector because it contains two antibiotic resistance genes that act as a selectable marker and recognition sites for restriction enzymes.
Is pBR322 present in E. coli?
Related Biology Videos Assertion : E. coli having pBR322 with DNA insert at BamHI site cannot grow in medium containing tetracyline. Reason : Recoginition site for BamHI is present in `ter^(R)` region of pBR22.
What is the most important features of pBR322?
The main characteristics of pBR322 are: Restriction sites: BamH I, Hind III, Sal I, Pvu I, Pvu II, Pst I, EcoR I, Cla I. Selectable marker: antibiotic resistance genes for ampicillin (ampR) and tetracycline (tetR) ORI: the origin of replication.
What are Ti and Ri plasmids?
Ti plasmid is found in the bacterium Agrobacterium tumefaciens. Ri plasmid is found in the bacterium Agrobacterium rhizogenes. They cause crown gall disease in dicots. They cause hairy root disease in monocots. Also see: Plasmids and Vectors.
What are the selectable markers in pBR322?
pBR322 contains two selectable markers, i.e. antibiotic resistance genes for ampicillin (ampR) and tetracycline (tetR). Further reading: Plasmid.
What is the size of the plasmid vector pBR322?
pBR322 DNA is a commonly used plasmid cloning vector in E. coli (1). The molecule is a double-stranded circle 4,361* base pairs in length (2).
What is the history of E coli plasmid pBR322?
pBR322 is a plasmid and was one of the first widely used E. coli cloning vectors. Created in 1977 in the laboratory of Herbert Boyer at the University of California, San Francisco , it was named after Francisco Bolivar Zapata , the postdoctoral researcher who constructed it.
Are the antibiotic-resistant genes on pBR322 transposable?
The antibiotic-resistant genes on pBR322 are not transposable. The vector pBR322 was constructed in order to have a plasmid with a single PstI site, located in the ampicillin-resistant gene (Apr), in addition to four unique restriction sites, EcoRI, HindIII, BamHI and SalI.
What is a plasmid vector used for?
pBR322 DNA is a commonly used plasmid cloning vector in E. coli. Our products are to be used for Research Use Only. They may not be used for any other purpose, including, but not limited to, use in humans, therapeutic or diagnostic use, or commercial use of any kind.