What is a Bioruptor?
The Bioruptor® uses a unique system to uniformely process multiple samples in sealed tubes of 0.5 ml to 50 ml capacity. The built-in cooling system (water cooler and Single Cycle Valve) ensures high precision temperature control resulting in higher quality samples.
What is chromatin shearing?
Chromatin shearing is one of the first critical steps in the ChIP-Seq workflow. Depending on your approach, you will either faithfully represent the biological scenario you wanted to study, or disrupt epitopes and introduce all sorts of bias so you won’t actually know what’s relevant downstream.
What is DNA shearing?
DNA shearing is an experimental process used to prepare DNA for analysis or other processing by the use of mechanical instruments to randomly cleave DNA. DNA is sheared to the desired fragment range. For instance, physical shearing can be done by probe sonication and nebulization.
Why is sonicator used?
Sonication is widely used in the laboratory to disperse nanotubes into the polymer matrix. This process utilizes ultrasound energy to agitate nanoparticles in the polymer matrix. It is usually carried out by an ultrasonic bath or a horn/probe which is also known as the sonicator.
How do you break DNA into fragments?
In the laboratory, restriction enzymes (or restriction endonucleases) are used to cut DNA into smaller fragments. The cuts are always made at specific nucleotide sequences. Different restriction enzymes recognise and cut different DNA sequences.
Can centrifugation shear DNA?
Centrifugal shearing: DNA can be sheared by the use of centrifugal force to move DNA through a hole of a specific size; the rate of centrifugation determines the degree of DNA fragmentation. This method is used for generation of fragments many kb in length.
Does sonication break molecules?
In addition, it converts an electrical signal into physical vibration that can break substances apart. Therefore these disruptions can mix solutions, accelerate the dissolution of a solid into a liquid. In DNA testing, sonication breaks the molecules and ruptures cells and hence releasing proteins for testing.
Does sonication fragment DNA?
This protocol describes a method for DNA fragmentation by sonication. During sonication, DNA samples are subjected to hydrodynamic shearing by exposure to brief periods of sonication. DNA that has been sonicated for excessive periods of time is extremely difficult to clone.
Which technique is used to separate DNA fragments by their size?
Electrophoresis
Electrophoresis is a laboratory technique used to separate DNA, RNA or protein molecules based on their size and electrical charge.
What is DNA sonication?
During sonication, DNA samples are subjected to hydrodynamic shearing by exposure to brief periods of sonication. DNA that has been sonicated for excessive periods of time is extremely difficult to clone.
Can I Vortex DNA?
It’s safe to vortex some DNA samples, depending on what they are. Isolated PCR products or cDNA samples can generally be vortexed without damaging them. You should not vortex plasmids (especially big ones) or genomic DNA, because vortexing will shear long pieces of DNA into smaller fragments.
What is ultrasonic degassing?
The process of removing gases from ultrasonic cleaning machines is called degassing. Any water that comes from a pressurized water supply will naturally contain dissolved gases, and therefore the water will need to be degassed when first dispensed.
Why choose bioruptor ® plus sonication device?
“Very easy to use, no worries about the heating, high throughput.” “Easy setup and straightforward to operate. Great technology in that it keeps your samples…” “Excellent instrument for DNA shearing from tissue. Had attempted using a sonicator which d…” You are about to request a quote for Bioruptor ® Plus sonication device.
Can I request a quote for bioruptor ® Pico sonication device?
You are about to request a quote for Bioruptor ® Pico sonication device. Fill out the form below and we will be in touch with you very soon. The Bioruptor® Pico is the latest innovation in shearing and represents a new breakthrough as an all-in-one shearing system capable of shearing samples from 150 bp to 1 kb.
What happens if the sonication in my ChIP assay fails?
Sonication is a critical first step in most ChIP assays. If the sonication in your ChIP assay is not efficient or does not generate enough chromatin, there is nothing you can do to save the experiment, no matter how good the ChIP antibody is or how robust the rest of the ChIP protocol is.
What is a perfect sonication profile for Chip?
A perfect sonication profile is a profile in which the highest specific signal and the lowest background are generated. There is no one universal size range generally applicable for ChIP. As a rule, fragments between 100-500 bp are suitable for the majority of ChIP experiments and can be used as a starting point.