How does anti-GFP antibody work?
Anti-GFP antibody (ab290) is a highly versatile antibody that gives a stronger signal than other anti-GFP antibodies available. On Western blot the antibody detects the GFP fraction from cell extracts expressing recombinant GFP fusion proteins and has also been shown to be useful on mouse sections fixed with formalin.
Can GFP antibody detect EGFP?
The Full-Length GFP Polyclonal Antibody detects AcGFP1, wild type GFP, and GFPuv. Based on their homology to GFP, we also expect this antibody to detect EGFP, EYFP, and ECFP.
What is GFP Nanobody?
The green fluorescent protein (GFP)-nanobody is a single-chain VHH antibody domain developed with specific binding activity against GFP and is emerging as a powerful tool for isolation and cellular engineering of fluorescent protein fusions in many different fields of biological research.
What is zsGreen?
ZsGreen1 is the brightest commercially available green fluorescent protein—up to 4X brighter than EGFP—and is ideally suited for whole-cell labelling, promoter-reporter studies, or as a transfection control. Living Colors ZsGreen1 is an exceptionally bright green fluorescent protein derived from a Zoanthus sp.
How many BP is GFP?
730 bp
The GFP cDNA consists of 730 bp, which encode a 238 amino acid protein with a molecular weight of 27 kD (2). Wild-type GFP emits a vibrant green fluorescence upon exposure to blue light (450-490 nm). The signal is detectable by fluorescence microscopy and fluorescence-activated cell sorting (FACS) (3).
Can Nanobody enter cells?
A nanobody-peptide pair directly enters the cell cytosol, where the nanobody can bind its antigen.
What is GFPuv?
Living Colors GFP and GFPuv are bacterial expression vectors that carry the gene for either GFP (wild-type Aequorea victoria green fluorescent protein) or GFPuv (a GFP variant optimized for maximal fluorescence when excited by UV light described by Crameri et al.
What is mCherry antibody?
mCherry is a fluorescent protein derived from Discosoma sp. Mushroom corals.
What is antibody screening?
Antibody Screen. Group O red cells are used to avoid interactions with ABO antibodies. Any incompatibility with the screen cells should be due to antibodies other than normally occurring ABO antibodies. Antibody screens are done on recipients, as mentioned, but many forget the requirement that they are also performed on every blood donor!
What is the CPT code for antibody screen?
If Antibody Screen is positive, then Antibody Identification, Titer and Antigen Typing will be performed at an additional charge (CPT code (s): 86870 (x) # of panel (s) performed, 86886 (x) # of titer (s) performed and 86905 (x) # of antigen typing (s).
What happens if the antibody screen is negative?
Antibody Screen. The test can be done using one of several platforms, primarily either tubes, gel testing, or solid-phase testing. If the antibody screen is positive, in most cases the next step would be to perform antibody identification. If the screen is negative, there is a very high likelihood that no significant antibodies are present…
Why are group O red cells used for antibody screening?
Two important things to remember about antibody screening: Group O red cells are used to avoid interactions with ABO antibodies. Any incompatibility with the screen cells should be due to antibodies other than normally occurring ABO antibodies.