What does Weigert stain stain?
Definition. The Weigert stain involves the treatment of nervous tissue with potassium dichromate to preserve myelin lipids. The lipids are then stained with the use of hematoxylin, providing a useful technique for visualizing fiber pathways. The pathways appear black due to the staining of myelin sheaths.
Which stain is used for connective tissue?
Mason’s Trichrome stain is used to highlight connective tissue fibers in a tissue section. Collagen fibers are stained a blue hue, nuclei a blue-black, and cytoplasm, keratin, and muscle fibers a range of pink to red hues.
What does Weigert haematoxylin stain?
Weigert′s iron hematoxylin kit – for nuclear staining in histology is a ready to use staining kit used for human-medical cell diagnosis and serves the purpose of the histological investigation of sample material of human origin, for example histological sections of e. g. the liver, the kidney, the intestine, the …
What is Weigert haematoxylin?
A nuclear staining solution containing, hematoxylin, ferric chloride and hydrochloric acid used in many non-routine techniques (special stains) because, it resists decolorization in acidic staining solutions.
What is EVG stain?
PURPOSE: This stain is useful in demonstrating atrophy of elastic tissue in cases of emphysema, and the thinning and loss of elastic fibers in arteriosclerosis, and other vascular diseases.
What type of stain is Van Gieson stain?
Van Gieson’s stain is a mixture of picric acid and acid fuchsin. It is the simplest method of differential staining of collagen and other connective tissue. It was introduced to histology by American neuropsychiatrist and pathologist Ira Van Gieson.
What does Orcein stain?
Orcein stain is a staining technique used for the visualization of hepatitis B surface antigens (HBsAg), elastic fibers and copper-bound proteins. Viral particles present inside host cells are called viral inclusion bodies.
How do you make Weigert hematoxylin?
Short procedure
- Mix equal parts of Reagent A and B.
- Place the sample in the freshly prepared solution for 1-2 hours.
- Rinse with tap water.
- Add 1% HCl in 70% ethanol.
- Wash with tap water and then with distilled water.
- Dehydrate with graded ethanol, clear and coverslip.
What does Harris haematoxylin stain?
Product Description. The Harris Haematoxylin Stain (Ortho) is a specially formulated nuclear stain designed to stain nuclei of cytological preparations. The stained cell sample is used to facilitate the diagnosis of the pathological state intended for in-vitro examination of cellular deformity.
What is Congo red stain used for?
Staining with Congo Red (CR) is a qualitative method used for the identification of amyloids in vitro and in tissue sections.
Which stain is best for glycogen?
The selective staining of glycogen by Best’s Carmine and the other dyes used in a similar manner (e.g. Alizarin Brilliant Blue BS, Alizarin Red S, Gallein and Haematoxylin) is due to hydrogen bonding between phenolate (−O−) groups of the dye and glycogen hydroxyl groups.
Why is Aceto-orcein stain used?
Treatment with acid and heat is used to break up the cellulose cell wall allowing stain to permeate the tissue and makes it easier to squash the tissue on a microscope slide. Aceto-orcein stain turns chromosomes a purple-red colour.
What does acetic Orcein stain?
Acetic orcein staining of polytene chromosomes was introduced in 1941 shortly after the initial studies on aceto-carmine-stained chromosomes by Bridges (2) and has remained a standard method of preparation.
What type of stain is Congo red?
It is an azo dye.
What is fibrin glue?
Fibrin glue is a topical biological adhesive, the effect of which imitates the final stages of coagulation. The glue consists of a solution of concentrated human fibrinogen which is activated by the addition of bovine thrombin and calcium chloride. The resultant clot aids haemostasis and tissue seal … Fibrin glue Blood Rev.
What is a fibrin stain?
This is a Gram stain which is differentiated with aniline-xylene and is often done after prestaining with eosin or some other red counterstain. Fibrin retains the crystal violet, appearing deep blue on a pink background. Again, this technique is more successful with larger deposits than the erythrocyte sized ones sometimes encountered.
How effective is fibrin glue in peritoneal dialysis?
The use of fibrin glue in PD has been reported to be both effective in treating established leaks and, when used at the time of catheter implantation, may help prevent the development of initial peritoneal leaks.55–57 Our experience with fibrin glue would support both of these assertions.
What is the trichrome method of staining fibrin?
Perhaps the “type” trichrome method, as far as fibrin is concerned, is the Picro-Mallory. This technique uses three staining solutions and a polyacid to produce yellow erythrocytes, red fibrin and blue collagen.