How do you reconstitute primers IDT?
To obtain a 100 µM solution, multiply # nmol x 10. That will equal the # µL to use for resuspension. For example: 20 nmol X 10 = 200 µL.
Can you vortex oligos?
If resuspension is difficult, try heating the oligo at 55°C for 1–5 minutes, then vortex thoroughly.
Are IDT oligos single-stranded?
Product details. DNA oligos are short, single- and double-stranded synthetic DNA sequences that can be used in nearly any molecular biology application.
How is oligo concentration calculated?
Making a 100 μM solution To resuspend your oligonucleotides to 100 μM, simply multiply the number of nanomoles (nmol) by 10 to get the volume (in μL) of water or buffer to add. The equation on the left shows an example, assuming you have an oligonucleotide of 1 nmol final yield.
Can I dissolve primers in water?
I always dissolve PCR primers with double distilled water.
How can I tell if my oligos successfully annealed?
You can verify if your oligos successfully annealed by running them on a 2% non-denaturing PAGE gel with appropriate molecular weight markers, side by side with single-stranded oligo, or using a stain to visualize the bands.
What temperature should oligos be stored at?
We ship most of our oligos dry, and the short shipping time will not jeopardize oligo stability for any standard oligo. As mentioned earlier, dry oligos remain stable for up to 25 weeks when stored at 37°C (98°F).
Is oligo double-stranded?
Oligonucleotides or oligos are short single-stranded or double-stranded polymers of nucleic acids.
Can oligos be double stranded?
Yes, IDT can synthesize duplex oligos. When entering your oligo, select the Duplex option on the order form. Selecting this option will load a revised order form with space for the sequence of a complementary strand.
Are DNA oligos single or double stranded?
Most DNA molecules exist as a double helix made up of two complementary strands. Ribonucleic acid (RNA) oligonucleotides contain adenosine (A), guanosine (G), uridine (U), and cytidine (C) monomers linked by 3′,5′-phosphodiester bonds.
What does the prefix oligo mean?
just a few or scanty
Oligo- (prefix): Means just a few or scanty. From the Greek “oligos’, few, scanty. Examples of terms starting with oligo- include oligodactyly (few fingers), oligohydramnios (too little amniotic fluid) and oligospermia (too few sperm).
How are oligonucleotides synthesized?
In solid-phase synthesis, an oligonucleotide being assembled is covalently bound, via its 3′-terminal hydroxy group, to a solid support material and remains attached to it over the entire course of the chain assembly.
How do you calculate resuspension?
To resuspend your oligonucleotides to 100 μM, simply multiply the number of nanomoles (nmol) by 10 to get the volume (in μL) of water or buffer to add. The equation on the left shows an example, assuming you have an oligonucleotide of 1 nmol final yield.
How do you dissolve oligo?
Always briefly centrifuge oligos before opening for the first time. We dissolve the stock oligo in sterile dH2O which must be freshly autoclaved. Alternatively, TE buffer (10 mM Tris pH 8.0, 1 mM EDTA) can be used. For convenience, make a freezer stock at 100 µM concentration (which should be thawed infrequently).