What is gamma H2AX antibody?
Gamma H2AX (gammaH2AX) is the phosphorylated version of histone H2AX and is a marker for double-stranded breaks (DSBs) caused by DNA damage (1-4).
What is phospho H2AX?
The phospho-H2AX (Ser139) kit offers quantitative detection of the histone variant H2AX, phosphorylated on serine 139. This specific phosphorylation may occur when double-stranded DNA breaks, either due to ionizing radiation or to endogenous physiological processes.
Why it is called Gamma H2AX?
H2AX is a variant of the H2A histone. When DNA undergoes double-strand break damage, H2AX is phosphorylated by ATM (or ATR). This phosphorylation of H2AX is named gamma-H2AX (y-H2AX).
What is the function of gamma H2AX?
This modified form, termed γ-H2AX, is easily identified with antibodies and serves as a sensitive indicator of DNA DSB formation.
What is Gamma H2AX a marker of?
What is RAD51 mutation?
RAD51 mutation was found in individuals with actual congenital mirror movements. Variants of the DNA damage repair gene RAD51 increase the risk of esophageal cancer. BRG1-RAD52 complex mediates the replacement of RPA with RAD51 on single-stranded DNA (ssDNA) to initiate DNA strand invasion.
What is comet assay used for?
The comet assay (single-cell gel electrophoresis) is a simple method for measuring deoxyribonucleic acid (DNA) strand breaks in eukaryotic cells. Cells embedded in agarose on a microscope slide are lysed with detergent and high salt to form nucleoids containing supercoiled loops of DNA linked to the nuclear matrix.
What does comet assay detect?
Comet assay is a microgel electrophoresis technique, which detects DNA damage and repair in individual cells. The assay measures DNA damage (i.e., strand breaks, DNA adducts, excision repair sites, and cross-links) at the single-cell level.
What is H2AX antibody?
Antibodies that detect Histone H2A.X can be used in several scientific applications, including Western Blot, Immunocytochemistry, Immunohistochemistry (Paraffin), Flow Cytometry View more Histone H2A.X (H2AX) is a member of the histone H2A family which is one of the four core histones making up the nucleosome core particle.
What is phospho-h2a X Antibody?
Phospho-H2A.X (Ser139) Antibody detects endogenous levels of H2A.X only when phosphorylated at Ser139. Antibodies are produced by immunizing animals with a synthetic phosphopeptide corresponding to residues surrounding Ser139 of human H2A.X.
What is the H2AX variant?
H2AX is a variant of histone H2A, one of the histone core molecules forming the nucleosome, and is a vital component in repairing DNA damage (1-4). The H2AX variant represents between 2-25% of total H2A (1-3).
What is gamma histone H2AX?
Histone H2A.X (H2AX) is a member of the histone H2A family which is one of the four core histones making up the nucleosome core particle. In eukaryotes, DNA double strand breaks (DSBs) have been shown to trigger the phosphorylation of serine 139 at the carboxy terminus of histone H2AX resulting in gamma-H2AX.