What is VSV-G protein?
Vesicular stomatitis virus G (VSV G) protein is a typical type III viral fusion protein. To study the mechanism of VSV G protein mediated membrane fusion, we set up a cell-cell fusion system in which cells are marked by different fluorescent proteins.
What is VSV-G used for?
VSV-G is a trimeric protein that binds phosphatidylserine and low-density lipoprotein receptors (LDLRs) on a cell surface to endocytose into the cell. The reason VSV-G is so commonly used is that it is good at infecting most cells, but not all.
What is VSV-G plasmid?
VSV-G envelope glycoprotein forms complexes with plasmid DNA and MLV retrovirus-like particles in cell-free conditions and enhances DNA transfection. Mol Ther.
How does VSV-G enter cells?
VSV enters the cell via the endocytic pathway and subsequently fuses with a cellular membrane within the acidic environment of the endosome.
What kind of virus is VSV?
Vesicular stomatitis virus (VSV) is an enveloped, negative-sense RNA virus that infects a wide variety of mammalian and insect cells. Infections in humans are asymptomatic or result in a mild febrile illness. This virus is also exquisitely sensitive to interferons (IFN).
What is VSV disease?
Vesicular stomatitis is a viral disease which primarily affects horses, cattle, and swine. The agent that causes vesicular stomatitis, VSV, has a wide host range and can occasionally infect sheep and goats.
What type of vaccine is VSV?
The recombinant VSV (rVSV) platform was developed by John Rose and Michael Whitt9,10. rVSV was previously developed as a vaccine platform for several viral pathogens, including Ebola virus (EBOV), human immunodeficiency virus, and Crimean–Congo hemorrhagic fever virus11,12.
What is vesicular stomatitis virus glycoprotein?
Abstract. Glycoprotein G of the vesicular stomatitis virus (VSV) is involved in receptor recognition at the host cell surface and then, after endocytosis of the virion, triggers membrane fusion via a low pH-induced structural rearrangement.
What is the receptor for VSV G?
Vesicular stomatitis virus (VSV) is an oncolytic rhabdovirus and its glycoprotein G is widely used to pseudotype other viruses for gene therapy. Low-density lipoprotein receptor (LDL-R) serves as a major entry receptor for VSV.
What does VSV infect?
The agent that causes vesicular stomatitis, VSV, has a wide host range and can occasionally infect sheep and goats. In affected livestock, VSV causes blister-like lesions to form in the mouth and on the dental pad, tongue, lips, nostrils, hooves, and teats.
How do you treat VSV in horses?
No specific treatment is needed. Cleaning ulcers with mild antiseptics may help avoid bacterial infections. To reduce the risk of exposure to the virus, owners may limit pasture time, provide shelters or barns during insect feeding times, and take other steps to reduce animal contact with insects.
How do VSV vaccines work?
VSV-based vaccines can mount both strong humoral (6) and potent cellular immune responses against pathogens (7). Accordingly, VSV vectors have been shown to induce protective immunity against a large number of different pathogens in animal models, e.g., HIV, influenza virus, Marburg virus, and Ebola virus (8–10).
Is VSV a DNA virus?
VSV is another single-stranded RNA virus of the rhabdoviridae family, which includes the rabies virus. Because it is closely related to rabies in structure and biology, yet less pathogenic, it has been studied as a proxy to understanding rabies biology.
How do you treat vesicular stomatitis virus?
There is no specific treatment or cure for vesicular stomatitis. Good sanitation and quarantine practices on affected farms usually contain the infection. When definite diagnosis is made on a farm, work with the State Veterinarian’s office to determine necessary quarantine procedures.
Is there a vaccine for vesicular stomatitis in horses?
Vaccines have been developed experimentally to help combat vesicular stomatitis, but none are currently approved for use in horses. There is considerable debate over the efficacy of vaccination in preventing or reducing the severity of an outbreak.
What is VSV based vaccine?
Vesicular stomatitis virus (VSV), a negative-strand RNA virus of the rhabdovirus family, is a potent candidate vaccine vector. VSV-based vaccines can mount both strong humoral (6) and potent cellular immune responses against pathogens (7).
What is the incubation period for vesicular stomatitis?
Clinical Signs The incubation period for vesicular stomatitis is 2 to 8 days. The first sign of illness is often excessive salivation.
What is the function of VSV G protein?
These proteins mediate fusion between the virus envelope and host cellular membrane to release the viral genome into cells. Vesicular stomatitis virus G (VSV G) protein is a typical type III viral fusion protein.
What is vesicular stomatitis virus G glycoprotein (VSV-G)?
Production of vesicular stomatitis virus G glycoprotein (VSV-G) pseudotyped retroviral vectors Retrovirus pseudotype is defined as the genome of one retrovirus encapsidated by the envelope protein of a second virus.
What is the genome of VSV?
VSV has an 11 kb genome that consists of a single strand of negative-sense RNA. VSV transmission in animals occurs through insect bites, and can cause severe disease in cattle, horses, and swine with symptoms similar to foot and mouth disease ( Rodriguez, 2002 ).
What does VSV G stand for?
Vesicular stomatitis virus G (VSV G) protein is a typical type III viral fusion protein. To study the mechanism of VSV G protein mediated membrane fusion, we set up a cell-cell fusion system in which cells are marked by different fluorescent proteins.